Research report on the content and solubility of potassium cinnamate

2021-02-02

　1、 Enterprise introduction
Wuhan Nengmeike Industrial Co., Ltd. is a manufacturer specializing in the production and sales of cinnamon series products and derivatives. Founded in 2012, the company has an independent research and development team, independent research and development capabilities and rich production experience. Its main products are: cinnamaldehyde, cinnamic acid, cinnamic alcohol, methyl cinnamate, potassium cinnamate, etc.
2、 Introduction to potassium cinnamate
1. Application mechanism
In recent years, potassium cinnamate has been applied in more and more fields, and more and more people have been used. It is mainly due to its antiseptic, bacteriostatic and fragrance enhancing functions, which are more than ten times of those of traditional preservatives, and it is cheap and cheap. Its antiseptic mechanism is that potassium cinnamate converts into cinnamic acid under acidic conditions. By inhibiting the use of phenylalanine and tyrosine by microorganisms, it is considered as a weak acid preservative that can be dissolved in microbial membranes and enter cells Ionization in a higher pH environment and therefore lowering the internal pH can effectively affect the activity of many important enzymes, thus ultimately killing the contaminated cells.
2. Our plant's synthesis process: food-grade cinnamic acid+food-grade potassium hydroxide+food-grade activated carbon chemical synthesis process.
There are also other synthesis processes on the market, roughly as follows: ① methyl acetate+styrene+carbon tetrachloride synthesis process. This process can be defined as industrial synthesis. The price of potassium cinnamate produced by this process will be much lower than that of the first process. Its raw material belongs to industrial grade chemical dangerous goods under control, and methyl acetate belongs to Class II dangerous chemicals. There will be a large amount of carbon tetrachloride residues in the produced products, which cannot be used as food additives. Carbon tetrachloride has a mild irritation on the mucous membrane, an anesthetic effect on the central nervous system, and serious damage to the liver and kidney. It is strictly prohibited to add carbon tetrachloride into food. ② The chemical synthesis process of cinnamic acid+potassium hydroxide+activated carbon, but the main raw materials are not food-grade standards. For example, potassium hydroxide will use industrial grade, the price will be low, and impurities and heavy metals will seriously exceed the standard, which is obviously not suitable for food additives.
3、 Comparison test of potassium cinnamate content
1. Tested products:
Potassium cinnamate (Nemco standard sample), potassium cinnamate 1 (market friend 1), potassium cinnamate 2 (market friend 2)
2. Test items:
Content detection
3. Test instrument:
Liquid chromatograph
4. Test method:
Wavelength: 285nm
C18 column
Mobile phase: acetonitrile: 0.1% phosphoric acid solution 30:70
Flow rate: 0.5ml/min
1g sample dissolved in 10ml methanol solution
5. Test data:
Standard sample:
Potassium cinnamate No. 1:
Potassium cinnamate No. 2:
6. Test results:
① The content of Nenmec standard sample: 99.13%. The content of potassium cinnamate 1 and 2 did not meet the standard.
② Peak time:
The peak time of potassium cinnamate should be about 20 minutes at the flow rate of 1ml/min. About 35 minutes should be the peak time of cinnamic acid. And there are many peaks, indicating that No. 1 and No. 2 may have a complex situation, and they are not pure potassium cinnamate.
③ Solvent preparation:
1. The phenomenon of serious insolubility in No. 2 indicates that there is insolubility in it. According to the chromatogram, it can be temporarily inferred as unreacted or compounded cinnamic acid.
4、 Test method for solubility of potassium cinnamate
1. Experimental equipment:
Water, potassium cinnamate powder, three cups, dropper, spoon, citric acid reagent.
2. Objective:
The solubility of 8% potassium cinnamate and 15% potassium cinnamate were tested respectively.
3. Experimental steps:
A. Pour different amounts of water into two cups, add potassium cinnamate continuously and stir, prepare 8% and 15% potassium cinnamate solution respectively, until the potassium cinnamate at the bottom of the container is completely dissolved.
B. Use a dropper to take a portion of the solution in the upper layer of the cup and put it into the sampling bottle.
C. Compare the dissolution of each sample.
4. Analysis:
A. 8% concentration of potassium cinnamate solution (cold water) is not dissolved and is turbid (as control group).
B. Potassium cinnamate solution with 8% concentration (water temperature 40~50 ℃) has good solubility and is a yellowish solution.
C. Dissolve the same amount of warm water at 40~50 ℃ with 15% concentration of potassium cinnamate solution again, and the solution will be pale yellow transparent liquid.
Comparison: 8% and 15% potassium cinnamate solution (hot water), and 15% potassium cinnamate solution are obviously darker in color.
5. Conclusion:
Therefore, it can be proved that the solubility of potassium cinnamate increases with the increase of temperature. In order to better dissolve it, it is necessary to continuously stir and raise the water temperature.
6. Remarks:
Take 10g of potassium cinnamate, add 60g of water to dissolve, heat slightly, prepare about 10% of citric acid, and add citric acid solution to precipitate cinnamic acid. If there is white crystalline solid precipitation, it is cinnamic acid, which proves that this potassium cinnamate is authentic. If there is no precipitation, it is possible that the product is not potassium cinnamate at all or the content is very low. The above detection methods can be used for detection.